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KMID : 1094020140310020095
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Journal of Veterinary Clinics
2014 Volume.31 No. 2 p.95 ~ p.101
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Effects of 0.1% Tacrolimus on Canine Skin Mast Cells and Eosinophils
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Jeong A-Young
Bae Seul-Gi
OH Tae-Ho
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Abstract
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Five dogs were used to determine whether 0.1% tacrolimus ointment application for one day would inhibitIgE-mediated late-phase reactions (LPRs). It was consisted of three periods: one period without therapeuticadministration (control) and two periods of treatment with either the tacrolimus ointment or vehicle. Induction of IgEmediatedLPRs was induced by intradermal injections of 0.05 ml (0.14 mg/ml) of solution of goat anti-canine IgEpolyclonal antibodies. Each section for mast cells (MCs) and eosinophils (EPs) was stained with acidified toluidineblue, and Luna’s stain, respectively. Assessment of anti-inflammatory effect of tacrolimus ointment composed of cellcounts of MC and EP from lesions of induced LPR. In normal canine biopsies, the number of dermal MCs and EPswere 12.3 ± 1.4 cells/mm2 and 3.1 ± 1.3 cells/mm2, respectively. MC counts dramatically decreased at time dependentmanner after anti-IgE administration. However, the number of MCs on 6 hours after challenge was significantly lessdecreased in the groups treated with the tacrolimus, as compared with control and vehicle group. The number of EPson 24 hours after challenge was significantly lower in the group treated with the tacrolimus than in the control andvehicle groups. In conclusion, this study revealed that 0.1% tacrolimus ointment in dogs may exert a potent antiinflammatoryeffect on inhibition of MC degranulation and also secondary prevention of EP infiltration during LPR.
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KEYWORD
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tacrolimus, mast cell, eosinophil, late-phase reaction, atopic dermatitis, dog
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